April – the season for the new school term.
Meet Maya Akutsu, a mysterious new teacher at Hanzaki Elementary School. Her way of talking and behaving is as graceful and magnificent as a queen. Miss Akutsu, with her high intelligence, artistic talent, and perfect athletic skills, soon overwhelms the other teachers and makes all the parents obedient. She takes charge of "Class Three" in the sixth grade. In Miss Akutsu's class, test scores precede everything else. She gives pop quizzes every week and students with low scores have to do odd jobs in the classroom, like slaves. If they try to disobey, they're severely punished. Miss Akutsu is the law. She is the queen of her domain.
Kazumi, one of her students, soon becomes her main target. Although Kazumi is a sweet and innocent twelve-year-old girl, she cannot achieve above average on either her exams or in gymnastics. Kazumi withstands Miss Akutsu's outrageous brand of “punishment,” but her classmates begin to crack in the grip of fear. Jealousy, slander, and betrayal run rampant in the class. Even in the darkest time of her life, however, Kazumi strongly wishes to “graduate happily with her classmates”, and she finally decides to gather up all the students to face their evil teacher.
How will the 24 sixth-graders grow up during the final year at elementary school? And what is Miss Akutsu's real purpose of being so devilish toward her students?

 四月份的新学期， 半崎小学新上任了一位名叫阿久津真矢的神秘女教师。

她言行举止，似女王般的优雅庄重。学识、艺术、运动所做的一切都完美无暇。为此她超越了其他老师，连学生的家长们对她都言听计从。真矢担任起六年级三班的班主任。在她的班级里，成绩就代表一切。每周考试，成绩差的学生将像奴隶一样被对待。如果有谁敢反抗，就会受到精神上的彻底惩罚。在班上她就是法律，就是女王！面临恶魔老师，六年级三班每天都笼罩着黑暗。

率直善良的和美，学习和体育成绩都在平均点以下，正是真矢老师的瞄准典型。和美默默地忍受着来自于真矢老师的几乎是不尽情理的「惩罚」。因为恐惧真矢老师的惩罚，班上开始出现嫉妒、猜忌、背叛、诽谤等等恶风气。乌烟瘴气的教室里， 只有和美还是执著地盼望着能与大家一起快乐地毕业。为了将人心涣散的班级再次重新聚拢起来，和美勇敢地站了起来，开始与真矢老师抗争。 

二十四个学生，最终变成了什么样子呢？而真矢老师的真正目的又何在呢？『女王的教室』刻画了迄今为止，以小学为舞台的电视连续剧中，最恶的女教师形象。以及与其抗争的二十四名小学六年级学生的一年的成长过程。